Journal: Neoplasia (New York, N.Y.)

Article Title: Use of DNA Microarray and Small Animal Positron Emission Tomography in Preclinical Drug Evaluation of RAF265, a Novel B-Raf/VEGFR-2 Inhibitor 1 2

doi:

Figure Lengend Snippet: Pathways Modulated by RAF265 in A375M Xenografts.

Article Snippet: Day 8 , 141 , Apoptosis , MetaCore GeneGo Processes , 131161 , Apoptosis_Apoptotic mitochondria , 85 , 3.44 e - 07 , 4.88 e - 06.

Techniques:

Journal: Oncology Research

Article Title: Anticancer efficacy of 3-(4-isopropyl) benzylidene-8-ethoxy, 6-methyl, chroman-4-one (SBL-060), a novel, dual, estrogen receptor-Akt kinase inhibitor in acute myeloid leukemia cells

doi: 10.32604/or.2022.03539

Figure Lengend Snippet: Functional cellular assays demonstrating the effect of SBL-060 in THP-1 and HL-60 cells. (a) Representative histograms of cell cycle analysis in both the differentiated AML cells with different SBL-060 dose treatments. A dose-based increase in the sub G 0 /G 1 phase of the cell cycle can be observed in both THP-1 and HL-60 cells at the end of 72 h. Data values are mean ± standard deviation (SD) percentage of sub G 0 /G 1 phase cells from different experiments. (b) Early and late phase apoptotic cell populations in THP-1 and HL-60 cells treated with different SBL-060 doses for 48 h. Representative plots are presented. The lower right quadrant indicates early apoptotic cells, and the upper right quadrant represents late apoptotic cells. Numerical values are the total apoptotic cells, presented as mean ± SD from different experiments.

Article Snippet: Data analysis was performed using InCyte software to differentiate between healthy and apoptotic cells (early and late apoptosis) and presented using GraphPad Prism version 6.0.

Techniques: Functional Assay, Cell Cycle Assay, Standard Deviation

Journal: Scientific Reports

Article Title: Blocking CCL5-CXCL4 heteromerization preserves heart function after myocardial infarction by attenuating leukocyte recruitment and NETosis

doi: 10.1038/s41598-018-29026-0

Figure Lengend Snippet: Analysis of infarcted area after MKEY treatment. Area at risk (AAR) as % total area ( A ) and infarcted area as % of AAR ( B ) were determined by Evans-blue perfusion and tetrazolium staining 1d after injury in control and MKEY-treated mice. ( C ) Representative heart sections from both groups are shown. *p < 0.05 vs. control (n = 6); ( D ) Quantification of the number of apoptotic cells as determined by TUNEL staining in MKEY- and control-treated mice. *p < 0.05 vs. control (n = 8); ( E , F ) Representative images of TUNEL staining of infarct tissue. Scale bar 50 µm. ( E ) TUNEL-positive cells are green, inset: control staining. ( F ) TUNEL-positive cells are red and cardiomyocytes are visualised using α-actinin (green). Nuclei were stained using DAPI (blue).

Article Snippet: Serial sections (3 sections per mouse, 400 µm apart) were stained to analyse the infarcted area for neutrophil content (specific esterase, Sigma, St. Louis, MO), neutrophil extracellular trap formation (H3cit, abcam 5103, Cambridge, UK), macrophage content (F4/80), lymphocytes (CD3, both Serotec, Oxford, UK) apoptotic cells (TUNEL, MEBSTAIN apoptosis kit II, MBL, Woburn, MA, USA) and CD31-positive capillaries (Santa Cruz, Santa Cruz, CA).

Techniques: Staining, TUNEL Assay